Antioxidant, antimicrobial activities and total flavonoid contents of Cirsium bulgaricum DC. leaf extracts

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In this study, the antioxidant activities of methanol and
acetone extracts of Cirsium bulgaricum DC. (Asteraceae)
were evaluated by five antioxidant assays, including
phosphomolybdate method, cupric ion reducing capacity
(CUPRAC), 2,2’-azinobis(3-ethylbenzothiazoline-6-
sulfonicacid)/persulfate) ABTS/Persulfate, N, N-dimethyl-pphenylenediamine
(DMPD) and a Ce(IV)-based reducing
capacity (CERAC) methods. The 80% methanol and acetone
extracts showed 0.005-6.16±0.03 mmol/g and 0.004-
5.02±0.02 [(trolox (TR), catechin (CT), epicatechin (EC),
rutin (RT), quercetin (QR), naringin (NG) in CUPRAC and
ABTS/Persulfate methods, respectively]. According to DMPD
methods, inhibition effect were showed 2.3%±0.02 for 80%
methanol and 4.6%±0.07 for acetone. Ascorbic acid equivalent
mmol (AA)/g extract in CERAC method was found to be
0.0834±0.09 for 80% methanol and 0.5622±0.05 for acetone.
Ascorbic acid equivalent μg ascorbic acid (AA) /mL extract
were found 807.6±0.07 for 80% methanol and 292.4±0.01 for
acetone in phpsphomolybdate method. In the addition to the
antioxidant activity of the extract, the total flavonoid content
was measured. Total flavonoid content of 80% methanol and
acetone extracts was found to be 105.5-645±0.04 μg/ml
quercetin and rutin equivalent. For the determination of
antibacterial activities Escherichia coli (E.coli) ATCC 25922,
Escherichia coli(E.coli) O157:H7 ATCC 33150, Salmonella
E n t e r i t i d i s(S.E n t e r i t i d i s ) AT C C 1 3 0 7 6 , Listeria
monocytogenes(L.monocytogenes) AT C C 7 6 4 4 ,
Staphylococcus aureus(S.aureus) ATCC 25923 bacteria were
used as test bacteria. Acetone extracts showed antibacterial
activity against all of the tested bacteria ranged between
17.00±1.72- 19.88±1.75 mm and 80% methanol extracts
showed antibacterial activity against all of the tested bacteria
ranged between 11.35±1.43-14.43±1.32 mm.
Keywords: Antioxidant Activity, Antimicrobial Activity,
Total Flavonoid Contents, Cirsium bulgaricum


Antioxidant Activity, Antimicrobial Activity

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